piezo1 f f mice  (Jackson Laboratory)

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: Piezo1 expression is upregulated in the lung epithelium during ventilator-induced lung injury. (A) Representative immunofluorescence images of lung sections from sham-operated control mice and mice subjected to 6 h of mechanical ventilation. Piezo1 protein (red) is constitutively expressed and colocalizes (yellow in merged panels) with the epithelial cell marker CK8 (green). Nuclei were counterstained with DAPI (blue). Scale bar, 100 µm. (B) Representative images of immunohistochemical staining for Piezo1 expression in lung epithelium from sham-operated mice and mice subjected to 6 h of mechanical ventilation. Scale bar, 20 µm (original magnification, ×400). (C) Representative haematoxylin and eosin-stained lung sections from sham-operated and HTV-ventilated mice. Scale bar, 50 µm. (D) Quantitative lung injury score was determined based on histological evaluation of alveolar congestion, haemorrhage, leukocyte infiltration and alveolar wall thickness, each graded from 0 (normal) to 3 (severe). Data are presented as the median (interquartile range); n=6 mice/group and Pvalues were determined by Mann-Whitney U test. Lung injury was further evaluated on the basis of (E) protein concentration, (F) cell number in BALF and (G) wet/dry weight ratio. (H) MPO activity in lung tissue. Proinflammatory cytokines in BALF were evaluated using the following ELISA kits: (I) IL-6, (J) TNF-α and (K) IL-β. (E-K) Data are presented as the mean ± SD, n=6 mice/group. Pvalues were determined by Student's t-test. *P<0.05, **P<0.01, ***P<0.001. BALF, bronchoalveolar lavage fluid; CK8, cytokeratin 8; HTV, high tidal volume; MPO, myeloperoxidase.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Expressing, Immunofluorescence, Control, Marker, Immunohistochemical staining, Staining, MANN-WHITNEY, Protein Concentration, Activity Assay, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: CS induces Piezo1 expression and epithelial injury markers in lung epithelial cells in vitro . (A) Representative western blot analysis of Piezo1 protein expression in lung epithelial cells under control (unstretched) conditions, and after 3 or 6 h of CS. (B) Semi-quantification of Piezo1 protein expression normalized to that of GAPDH. (C) Piezo1 mRNA expression in sham and stretched cells (after 3 and 6 h). (D) Representative fluorescence images of F-actin (phalloidin, green) and nuclei (DAPI, blue) in control and stretched cells. Scale bar, 20 µm. Proinflammatory cytokine levels of (E) IL-6, (F) TNF-α and (G) IL-1β protein levels in sham versus stretched cells. Data are presented as the mean ± SD, n=6 each. Pvalues were determined by oneway ANOVA. *P<0.05, **P<0.01, ***P<0.001. CS, cyclic stretch.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Expressing, In Vitro, Western Blot, Control, Fluorescence

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: Lung epithelial-specific Piezo1 deletion attenuates ventilator-induced lung injury. (A) Validation of Piezo1 knockout efficiency in the lung epithelium. Representative immunofluorescence images of lung sections from control and Piezo1 CKO mice showing the expression of the Piezo1 protein (red), the epithelial cell marker CK8 (green), and nuclei (DAPI, blue). Scale bar, 50 µm. (B) Representative haematoxylin and eosin-stained lung sections from control and Piezo1 CKO mice after 6 h of mechanical ventilation. Scale bar, 50 µm. (C) Quantitative histopathological lung injury score. Data are presented as the median (IQR); n=6/group. Data were analysed using the Kruskal-Wallis test followed by Dunn's post hoc test. (D) Total protein concentration in BALF. (E) Total cell counts in the BALF. (F) Lung wet/dry weight ratio. (G) Lung MPO activity. BALF concentrations of the inflammatory cytokines (H) IL-6, (I) TNF-α and (J) IL-1β. (D-J) Data are presented as the mean ± SD, n=6 mice/group. Pvalues were determined by oneway ANOVA. *P<0.05, **P<0.01, ***P<0.001. BALF, bronchoalveolar lavage fluid; CK8, cytokeratin 8; CKO, conditional; HTV, high tidal volume; MPO, myeloperoxidase.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Biomarker Discovery, Knock-Out, Immunofluorescence, Control, Expressing, Marker, Staining, Protein Concentration, Activity Assay

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: Genetic inhibition of Piezo1 attenuates mechanical stretch-induced cytoskeletal disruption and the inflammatory response in lung epithelial cells in vitro . (A) Representative western blotting and (B) semi-quantitative analysis demonstrating efficient knockdown of Piezo1 protein expression in MLE-12 cells transfected with shPiezo1 compared with that in cells transfected with Scr-shRNA. (C) Piezo1 mRNA expression levels measured by quantitative PCR in cells transfected with shPiezo1 compared with those in cells transfected with Scr-shRNA. Proinflammatory cytokine levels in cell culture supernatants from Scr-shRNA and shPiezo1 cells under control conditions or after 6 h of CS: (D) IL-6, (E) TNF-α and (F) IL-1β protein levels. (G) Representative fluorescence images showing F-actin morphology (phalloidin, green) and nuclei (DAPI, blue) in Scr-shPiezo1 and shPiezo1 cells under control (unstretched) conditions or after 6 h of CS. Scale bar, 20 µm. Data are presented as the mean ± SD, n=6 each. Pvalues were determined by oneway ANOVA. **P<0.01, ***P<0.001. CS, cyclic stretch; Scr, scrambled; sh, short hairpin.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Inhibition, Disruption, In Vitro, Western Blot, Knockdown, Expressing, Transfection, shRNA, Real-time Polymerase Chain Reaction, Cell Culture, Control, Fluorescence

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: Piezo1 mediates CS-induced Ca 2+ influx in epithelial cells. (A) Quantitative analysis of cytosolic Ca 2+ levels (ΔF/F 0 ) measured using a microplate reader in Fluo-3 AM-loaded MLE-12 cells treated with the Piezo1 agonist Yoda1 (10 µM) for 0, 15 and 30 min. (B) Representative fluorescence microscopy images of Fluo-3 AM-loaded MLE-12 cells at 30 min following the onset of CS Scale bar, 50 µm. (C and D) Both genetic knockdown of Piezo1 (with shPiezo1) and pharmacological inhibition with GsMTx4 (5 µM) attenuated the Ca 2+ elevation induced by 30-min CS. (E) Genetic knockdown of Piezo1 (with shPiezo1) attenuated mechanical stretch-induced calcineurin activation after 6 h. (F-H) Pharmacological inhibition of calcineurin with CsA (10 µM) or FK506 (10 µM) significantly reduced mechanical stretch -induced cytokine production after 6 h. (I) Disruption of the F-actin network induced by 6-h mechanical stretching was reversed by pretreatment with CsA or FK506. Scale bar, 20 µm. Data are presented as the mean ± SD, n=6 each. Pvalues were determined by oneway ANOVA. *P<0.05, ***P<0.001. CS, cyclic stretch; CsA, cyclosporine A; Scr, scrambled; sh, short hairpin.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Fluorescence, Microscopy, Knockdown, Inhibition, Activation Assay, Disruption

Journal: Molecular Medicine Reports

Article Title: Mechanotransducer Piezo1 drives ventilator-induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway

doi: 10.3892/mmr.2026.13893

Figure Lengend Snippet: Piezo1 mediates CS-induced inflammation via calcineurin/NFATc3 signalling. (A) Immunofluorescence screening of the nuclear translocation of NFAT isoforms (NFATc1-4) upon CS. NFATc3 showed the most prominent nuclear translocation. Scale bar, 10 µm. (B) Representative western blot images showing NFATc3 protein levels in the nuclear and cytoplasmic fractions of MLE-12 cells transfected with Scr-shRNA or shPiezo1, with or without CS (6 h). GAPDH and Lamin B1 were used as loading controls for cytoplasmic and nuclear fractions, respectively. (C) Semi-quantitative analysis of cytoplasmic NFATc3 protein expression normalized to GAPDH. (D) Semi-quantitative analysis of nuclear NFATc3 protein expression normalized to Lamin B1. (E) Representative western blot images showing NFATc3 protein levels in the nuclear and cytoplasmic fractions of MLE-12 cells treated with or without calcineurin inhibitors (CsA, 10 µM; FK506, 10 µM) prior to CS (6 h). GAPDH and Lamin B1 were used as loading controls for cytoplasmic and nuclear fractions, respectively. (F) Semi-quantitative analysis of cytoplasmic NFATc3 protein expression normalized to GAPDH. (G) Semi-quantitative analysis of nuclear NFATc3 protein expression normalized to Lamin B1. Data are presented as the mean ± SD, n=6 each. Pvalues were determined by oneway ANOVA. *P<0.05, **P<0.01, ***P<0.001. CS, cyclic stretch; CsA, cyclosporine A; Scr, scrambled; sh, short hairpin.

Article Snippet: To establish male mice with tamoxifen-inducible, lung epithelial-specific deletion of Piezo1 ( Piezo1 CKO ), Piezo1 F/F mice ( Piezo1 tm2.1Apat/ J; Jackson Laboratory) were bred with Sftpc-Cre ERT mice (Jackson Laboratory) in our laboratory.

Techniques: Immunofluorescence, Translocation Assay, Western Blot, Transfection, shRNA, Expressing

Journal: Regenerative Therapy

Article Title: PIEZO1 activation enhances myogenesis and mitigates muscle degeneration in rotator cuff tear

doi: 10.1016/j.reth.2024.12.002

Figure Lengend Snippet: There were decreased myogenic differentiation ability and reduced expression of PIEZO1 in supraspinatus MuSCs after rotator cuff tears . a and b. Immunofluorescence staining and statistical analysis of MYHC for myogenically differentiated human MuSCs from healthy supraspinatus muscle (CTRL) or supraspinatus muscle with rotator cuff tears (RCT) (n = 5). Scale bar, 100 μm. c. The mRNA expression of MYH1, MYOG, and MCK of myogenically differentiated MuSCs in CTRL group and RCT group (n = 3). d. The mRNA expression of PIEZO1 and PIEZO2 of MuSCs in CTRL group and RCT group (n = 3). e. The protein levels of PIEZO1 in CTRL group and RCT group. ∗∗ indicated p < 0.01, ∗∗∗indicated p < 0.001.

Article Snippet: Wild-type male mice (C57BL/6, twelve weeks old) and Piezo1 f/f mice were obtained from Gem Pharmatech (Nanjing, China).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Regenerative Therapy

Article Title: PIEZO1 activation enhances myogenesis and mitigates muscle degeneration in rotator cuff tear

doi: 10.1016/j.reth.2024.12.002

Figure Lengend Snippet: Ablation of Piezo1 in MuSCs led to decreased myogenic differentiation ability. a. The mRNA expression of Piezo1 in Piezo1 flox/flox (f/f) MuSCs and Piezo1 knockout (KO) MuSCs (n = 3). b. The protein levels of Piezo1 in Piezo1 flox/flox (f/f) MuSCs and Piezo1 knockout (KO) MuSCs. c and d. Immunofluorescence staining and statistical analysis of MYHC for myogenically differentiated Piezo1 f/f MuSCs and Piezo1 KO MuSCs (n = 5). Scale bar, 50 μm. e. The mRNA expression of MYH1, MYOG, and MCK of myogenically differentiated Piezo1 f/f MuSCs and Piezo1 KO MuSCs (n = 3). ∗indicated p < 0.05, ∗∗ indicated p < 0.01, ∗∗∗indicated p < 0.001, ∗∗∗∗indicated p < 0.0001.

Article Snippet: Wild-type male mice (C57BL/6, twelve weeks old) and Piezo1 f/f mice were obtained from Gem Pharmatech (Nanjing, China).

Techniques: Expressing, Knock-Out, Immunofluorescence, Staining

Journal: Regenerative Therapy

Article Title: PIEZO1 activation enhances myogenesis and mitigates muscle degeneration in rotator cuff tear

doi: 10.1016/j.reth.2024.12.002

Figure Lengend Snippet: PIEZO1 regulated myogenic differentiation of MuSCs by MAPK pathways. a. KEGG enrichment analysis of differently expressed genes between human MuSCs from healthy supraspinatus muscle (CTRL) or supraspinatus muscle with rotator cuff tears (RCT). b. The protein levels of MEK, p-MEK, ERK, p-ERK, and GAPDH in MuSCs from healthy supraspinatus muscles after treatment with PIEZO1 agonist Yoda1 or inhibitor GsMTx4. c and d. Immunofluorescence staining and statistical analysis of MYHC for myogenically differentiated healthy MuSCs with treatment of MAPK signaling agonist TBHQ or inhibitor U0126 (n = 5). Scale bar, 50 μm. e. The mRNA expression of MYH1, MYOG, and MCK of myogenically differentiated healthy MuSCs with treatment of MAPK signaling agonist TBHQ or inhibitor U0126 (n = 3). ∗∗ indicated p < 0.01, ∗∗∗indicated p < 0.001.

Article Snippet: Wild-type male mice (C57BL/6, twelve weeks old) and Piezo1 f/f mice were obtained from Gem Pharmatech (Nanjing, China).

Techniques: Muscles, Immunofluorescence, Staining, Expressing

Journal: Regenerative Therapy

Article Title: PIEZO1 activation enhances myogenesis and mitigates muscle degeneration in rotator cuff tear

doi: 10.1016/j.reth.2024.12.002

Figure Lengend Snippet: PIEZO1 agonist Yoda1 rescued myogenic differentiation deficit in MuSCs after rotator cuff tears . a and b. Immunofluorescence staining and statistical analysis of MYHC for myogenically differentiated MuSCs after RCT with or without treatment of PIEZO1 agonist Yoda1 (n = 5). Scale bar, 50 μm. c. The mRNA expression of MYH1, MYOG, and MCK of myogenically differentiated MuSCs after RCT with or without treatment of PIEZO1 agonist Yoda1 (n = 3). ∗indicated p < 0.05, ∗∗ indicated p < 0.01, ∗∗∗indicated p < 0.001.

Article Snippet: Wild-type male mice (C57BL/6, twelve weeks old) and Piezo1 f/f mice were obtained from Gem Pharmatech (Nanjing, China).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Regenerative Therapy

Article Title: PIEZO1 activation enhances myogenesis and mitigates muscle degeneration in rotator cuff tear

doi: 10.1016/j.reth.2024.12.002

Figure Lengend Snippet: PIEZO1 agonist Yoda1 could alleviate the muscle degeneration and enhance the shoulder function in RCT model . a. The schematic diagram of animal experimental design. b and c. Immunofluorescence staining of Laminin and statistical analysis of cross-sectional area of myofibers in supraspinatus muscles with or without treatment of PIEZO1 agonist Yoda1 after rotator cuff tears (n = 6). Scale bar, 50 μm. d. The gait analysis for rotator cuff tears models with or without treatment of PIEZO1 agonist Yoda1 after rotator cuff tears (n = 6). e and f. Fatigue running time for rotator cuff tears models with or without treatment of PIEZO1 agonist Yoda1 after rotator cuff tears (n = 6). ∗indicated p < 0.05, ∗∗∗indicated p < 0.001.

Article Snippet: Wild-type male mice (C57BL/6, twelve weeks old) and Piezo1 f/f mice were obtained from Gem Pharmatech (Nanjing, China).

Techniques: Immunofluorescence, Staining, Muscles

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) PIEZO1 mRNA expression by qPCR in purified mouse retinas from P6 mice injected with 100 µg TAM at P4 (n=5). Error bars: SEM. **P-value < 0.01, Mann– Whitney U test. (B) IB4 (magenta) and PIEZO1 (white) staining of retinal flat mounts from postnatal day (P) 6 Alk1 f/f Cdh5 Cre ERT2 and Alk1 f/f pups. (C) PIEZO1 mRNA expression by qPCR in HUVECs transfected with Ctrl or ALK1 siRNA (n=3) (D) Western blot analysis HUVECs transfected with Ctrl or ALK1 siRNA after 72 hr. (E) Quantifications of PIEZO1 levels normalized to β-ACTIN (n=4) (F) Immunostaining of healthy skin adjacent (ADJ) to telangiectasias and telangiectatic lesions (TE lesions) from patients with HHT type 2 for PIEZO1 (green), CD31 (magenta), and ENG (white). (G) Quantification of PIEZO1 staining (n=8; 4 patients, with 2 images selected per patient for analysis). Error bars: SEM. *P-value < 0.05, Mann–Whitney U test. Scale bars: 500 μm (B) and 50 μm (F).

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Expressing, Purification, Injection, MANN-WHITNEY, Staining, Transfection, Western Blot, Immunostaining

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) Schematic representation of the experimental strategy for Alk1 and Piezo1 deletion in mice from P4 to P6. (B) IB4 staining of retinal flat mounts from Alk1 f/f controls, Alk1 f/f Mfsd2a Cre ERT2 , Alk1 f/f Piezo1 f/+ Mfsd2a Cre ERT2 , and Alk1 f/f Piezo1 f/f Mfsd2a Cre ERT2 P6 mice. (C) Quantification of AVM number. n=10-12 mice per group. One-way ANOVA with Sidak’s multiple comparisons test. (D) Quantification of AVM vessel diameter (n=10-12). One-way ANOVA with Sidak’s multiple comparisons test. (E and F) Vascular labeling with latex dye (blue) in the retinas and gastrointestinal (GI) tract of Alk1 f/f and Alk1 f/f Cdh5 Cre ERT2 , Alk1 f/f Piezo1 f/+ Cdh5 Cre ERT2 , and Alk1 f/f Piezo1 f/f Cdh5 Cre ERT2 P6 pups. Black arrows indicate AVMs. (G) Experimental strategy to assess the effects of PIEZO1 inhibitor in Alk1 deleted retinas. Arrowheads indicate the time course of TAM (100 μg) and GsMTx4 (1 mg/kg) or vehicle administration. (H) IB4 staining of P6 retinal flat mounts from Alk1 f/f Cdh5 Cre ERT2 mice injected with GsMTx4 or vehicle at P4 and P5. ‘A’ indicates arteries, ‘V’ indicates veins, and arrows denote AVMs. (I) Quantification of AVM count. Each dot represents one retina (n=10). Mann–Whitney U test. Error bars: SEM. *P-value < 0.05, **P-value < 0.01, *** P-value < 0.001, ns: nonsignificant, Scale bars: 500 μm (B and H), 200 μm (E), 10 mm (F)

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Staining, Labeling, Injection, MANN-WHITNEY

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) Western blot analysis of HUVECs transfected with control, ALK1 , PIEZO1 , and ALK1 + PIEZO1 siRNAs, followed by 45 min of flow exposure. (B-D) Quantification of P-VEGFR2/VEGFR2, P-ERK5/ERK5 and ERK5/β-ACTIN. One-way ANOVA with Sidak’s multiple comparisons test. (E) ERK5 staining for siCon , siALK1 and siALK1 + siPIEZO1 transfected HUVECs under static conditions and after 16 hr of flow exposure. ERK5 (green) DAPI (blue) (F) Quantification of nuclear ERK5 translocation for siCon , siALK1 and siALK1 + siPIEZO1 transfected HUVECs under static conditions and following 16 hr of flow exposure. *P<0.05, ***P<0.001, ns: nonsignificant, One-way ANOVA with Sidak’s multiple comparisons test. (G) p62 immunostaining in siCon , siALK1 and siALK1 + siPIEZO1 transfected HUVECs under static conditions and following 16 hr of flow exposure. (H) Quantification of p62 expression in siCon , siALK1 and siALK1 + siPIEZO1 transfected HUVECs under static conditions and after 16 hr of flow exposure. One-way ANOVA with Sidak’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ns: nonsignificant, Scale bars: 25 μm (E and G)

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Western Blot, Transfection, Control, Staining, Translocation Assay, Immunostaining, Expressing

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) KLF4 (white) and IB4 (green) staining of retinal flat mounts from P6 Alk1 f/f , Alk1 f/f Mfsd2a Cre ERT2 and Alk1 f/f Piezo1 f/f Mfsd2a Cre ERT2 pups. (B) KLF4 immunostaining in siCon , siALK1 and siALK1 + siPIEZO1 transfected HUVECs under static conditions and after 16 hr of flow exposure. KLF4 (magenta), DAPI (blue) (C) Quantification of nuclear KLF4 in HUVECs transfected with siCon, si ALK1 , or si ALK1 +si PIEZO1 under static conditions and following 16 hr of flow exposure. One-way ANOVA with Sidak’s multiple comparisons test. (D) Western blot analysis of HUVECs transfected with control, ALK1 , PIEZO1 , and ALK1 + PIEZO1 siRNAs, followed by 16 hr of flow exposure. (E and F) Quantification of KLF4/β-ACTIN and P-AKT/AKT. One-way ANOVA with Sidak’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ns: nonsignificant, Scale bars: 250 μm (A) and 25 μm (B)

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Staining, Immunostaining, Transfection, Western Blot, Control

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) Immunostaining of healthy skin adjacent (ADJ) to telangiectasias and telangiectatic lesions (TE lesions) from patients with HHT type 2 for KLF4 (magenta), PIEZO1 (green), and CD31 (white). (B) Quantification of KLF4 staining (n=6; 3 patients, with 2 images selected per patient for analysis). Error bars: SEM. *P-value < 0.05, Mann–Whitney U test. Scale bar: 50 μm (A)

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Immunostaining, Staining, MANN-WHITNEY

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) Area of retinal hypoxia 90 min after pimonidazole injection in Alk1 f/f , Alk1 f/f Cdh5 Cre ERT2 and Alk1 f/f Piezo1 f/f Cdh5 Cre ERT2 P6 pups. Hypoxyprobe (green), IB4 (magenta) (B) Immunostaining of HIF1A (white) and IB4 (green) in retinal flat mounts from P6 Alk1 f/f , Alk1 f/f Mfsd2a Cre ERT2 and Alk1 f/f Piezo1 f/f Mfsd2a Cre ERT2 mice. Scale bars: 500 μm (A and B),

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Injection, Immunostaining

Journal: bioRxiv

Article Title: PIEZO1 overexpression in hereditary hemorrhagic telangiectasia arteriovenous malformations

doi: 10.1101/2024.11.27.625696

Figure Lengend Snippet: (A) Labeling for EdU (green), ERG (white), and CD31 (magenta) in the vascular plexus of retinas from P6 Alk1 f/f and Alk1 f/f Mfsd2a Cre ERT2 and Alk1 f/f Piezo1 f/f Mfsd2a Cre ERT2 mice. (B) Quantification of the number of ERG and EdU double positive nuclei per vascular area. Mann–Whitney U test. (C) Labeling for EdU (green) and Hoechst33342 (blue) in HUVECs transfected with control, ALK1 , PIEZO1 , and ALK1 + PIEZO1 siRNAs. (D) Quantification of the number of EdU and Hoechst33342 double positive nuclei (n = 5). Two-way ANOVA with Tukey’s multiple comparisons test. Error bars: SEM. **P-value < 0.01, ***P<0.001, ns: nonsignificant, Scale bars: 500 μm (A), 25 μm (C)

Article Snippet: Piezo1 f/f mice were purchased from the Jackson Laboratory (Strain #:029213).

Techniques: Labeling, MANN-WHITNEY, Transfection, Control